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Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Article DOI: 10.3791/60117-v • 09:14 min • October 20th, 2019
October 20th, 2019 •
1,2, 1, 1, 3, 1,4
1MRC Center for Regenerative Medicine, University of Edinburgh, 2Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, 3Italian Image Institute, 4Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine, University of California

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Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization.

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Human Adipose Tissue Micro-fragmentation Cell Phenotyping Secretome Characterization Regenerative Cells Microvascular System In-vivo Transplantation In-vitro Culture Cell Isolation Enzyme-free Processing Micro-architecture Microvasculature Cell Therapy Product Closed System Technology Harvesting Processing Re-injection Refined Fat Tissue Cosmetics Orthopedics Proctology Gynecology Tissue Regeneration
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