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Journal / Cancer Research / 3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统…
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JoVE Journal Cancer Research
3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统
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3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统

Article DOI: 10.3791/60122-v • 06:27 min • August 8th, 2019
August 8th, 2019 •
1,2, 1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 2Instituto de Medicina Molecular, Lisbon

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该协议的目标是建立一个3D体外模型,以研究肿瘤状环境中癌症相关成纤维细胞(CAFs)的分化,可在免疫荧光、转录等不同分析系统中解决。分析和生命细胞成像。

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癌症研究 问题 150 球体 CAF 3D 模型 分化 免疫荧光染色 qPCR 流细胞学 入侵 细胞外基质凝胶
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