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JoVE Journal Cancer Research
En 3D Spheroid model som et mere fysiologisk system for kræft-associeret fibroblaster differentiering og invasion in vitro undersøgelser
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En 3D Spheroid model som et mere fysiologisk system for kræft-associeret fibroblaster differentiering og invasion in vitro undersøgelser

Article DOI: 10.3791/60122-v • 06:27 min • August 8th, 2019
August 8th, 2019 •
1,2, 1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 2Instituto de Medicina Molecular, Lisbon

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Målet med denne protokol er at etablere en 3D in vitro-model til at studere differentieringen af Cancer-associerede fibroblaster (CAFs) i en tumor bulk-lignende miljø, som kan behandles i forskellige analysesystemer, såsom immunofluorescens, transkriptional analyse og Life Cell Imaging.

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Kræftforskning sfæroider CAF 3D model differentiering immunfluorescent farvning qPCR flow cytometry invasion ekstracellulær matrix gel
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