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Developmental Biology

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JoVE Journal Developmental Biology
Micro injectie van DNA in de Oogtoppen van Xenopus laevis embryo's en beeldvorming van GFP dat optische axonale Arbors uitdrukt in intact, levend Xenopus tadpoles
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Micro injectie van DNA in de Oogtoppen van Xenopus laevis embryo's en beeldvorming van GFP dat optische axonale Arbors uitdrukt in intact, levend Xenopus tadpoles

Article DOI: 10.3791/60123-v • 06:32 min • September 4th, 2019
September 4th, 2019 •
1, 1, 1
1College of Osteopathic Medicine, Touro University California

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Dit protocol is bedoeld om te laten zien hoe een DNA/DOTAP mengsel microinjecteert in de eyebuds van een dag oude Xenopus laevis embryo's, en hoe te beeld en reconstrueren individuele groene fluorescerende eiwitten (GFP) uitdrukken optische axonale Arbors in tectal midbrains van intact, levende Xenopus tadpoles.

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Ontwikkelingsbiologie uitgave 151 Xenopus laevis lipofection Optic axonale Arbors GFP Imaging micro Injection eyebuds
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