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Journal / Developmental Biology / Mikroinjektion af DNA i øjen knopper i xenopus…
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Developmental Biology

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JoVE Journal Developmental Biology
Mikroinjektion af DNA i øjen knopper i xenopus laevis embryoner og billeddannelse af Gfp udtrykker optiske Axonal Arbors i intakt, levende xenopus Tadpoles
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Mikroinjektion af DNA i øjen knopper i xenopus laevis embryoner og billeddannelse af Gfp udtrykker optiske Axonal Arbors i intakt, levende xenopus Tadpoles

Article DOI: 10.3791/60123-v • 06:32 min • September 4th, 2019
September 4th, 2019 •
1, 1, 1
1College of Osteopathic Medicine, Touro University California

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Denne protokol har til formål at demonstrere, hvordan man mikroinjicere en DNA/dotap blanding i eyebuds af en dag gamle xenopus laevis embryoner, og hvordan man billede og rekonstruere individuelle grønne fluorescerende protein (gfp) udtrykker optiske axonal Dorne i tectal midhjerner af intakt, levende Xenopus Tadpoles.

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Udviklingsmæssige biologi Xenopus laevis lipofection optiske axonal Arbors gfp Imaging mikroinjektion eyebuds
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