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JoVE Journal
Immunology and Infection

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Højeffektiv Transfection af primær makrofager med in vitro transkriberet mRNA
 
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Højeffektiv Transfection af primær makrofager med in vitro transkriberet mRNA

Article DOI: 10.3791/60143-v
November 9th, 2019

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Summary November 9th, 2019

Please note that all translations are automatically generated.

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Makrofager, især primær makrofager, er udfordrende at transficere, da de specialiserer sig i at opdage molekyler af ikke-selv oprindelse. Vi beskriver en protokol, der giver mulighed for yderst effektiv transfektering af primær makrofager med mRNA genereret fra DNA-skabeloner som plasmider.

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