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JoVE Journal
Immunology and Infection

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具有体外转录mRNA的初级巨噬细胞的高效转染
 
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具有体外转录mRNA的初级巨噬细胞的高效转染

Article DOI: 10.3791/60143-v
November 9th, 2019

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Summary November 9th, 2019

Please note that all translations are automatically generated.

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巨噬细胞,特别是原发性巨噬细胞,由于它们专门检测非自源分子,因此很难转染。我们描述了一种协议,它允许使用从DNA模板(如质粒)生成的mRNA对原性巨噬细胞进行高效的转染。

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