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JoVE Journal Developmental Biology
Isolering av specifika neuron populationer från spolmask Caenorhabditis elegans
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Isolering av specifika neuron populationer från spolmask Caenorhabditis elegans

Article DOI: 10.3791/60145-v • 09:42 min • August 6th, 2019
August 6th, 2019 •
1, 1, 1,2,3,4
1Department of Biochemistry, University of Nebraska, 2Nebraska Redox Biology Center, University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, 4Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

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Här presenterar vi ett protokoll för enkel isolering av specifika grupper av levande neuronala celler som uttrycker grönt fluorescerande protein från transgena Caenorhabditis elegans linjer. Denna metod möjliggör en mängd olika ex vivo studier fokuserade på specifika nervceller och har kapacitet att isolera celler för ytterligare kortsiktig odling.

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Utvecklingsbiologi utgåva 150 C. elegans cell isolering nervceller åldrande neuronala markörer grönt fluorescerande protein
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