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Journal / Cancer Research / 小鼠乳腺皮下HC11和EpH4细胞的分化
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Cancer Research

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JoVE Journal Cancer Research
小鼠乳腺皮下HC11和EpH4细胞的分化
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小鼠乳腺皮下HC11和EpH4细胞的分化

Article DOI: 10.3791/60147-v • 09:32 min • February 27th, 2020
February 27th, 2020 •
*1,2, *1, *1,3, 1,4, 1, 1, 2, 1
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, 2Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
* These authors contributed equally

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我们描述了两个乳房上皮线HC11和EpH4的分化诱导技术。虽然两者都需要胎儿小牛血清、胰岛素和乳素来产生牛奶蛋白，但EpH4细胞可以在三维培养中完全分化成乳腺球。这些互补模型对于分化和肿瘤的信号转导研究非常有用。

Tags

癌症研究，第156期，细胞分化，乳腺上皮细胞，乳素，EHS基质
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