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JoVE Journal Developmental Biology
Et svangerskap utenfor livmoren chemokine Expression modell for testing macrophage rekruttering in vivo
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Et svangerskap utenfor livmoren chemokine Expression modell for testing macrophage rekruttering in vivo

Article DOI: 10.3791/60161-v • 10:02 min • September 25th, 2019
September 25th, 2019 •
1, 1, 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

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For å teste effekten av en chemokine på macrophage rekruttering i Vivo, ble hele Mount in situ hybridisering brukt til å påvise chemokine, og immunostaining ble brukt til å merke makrofager. Live Imaging ble brukt for sanntids observasjon av macrophage migrasjon.

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Utviklingsbiologi hele Mount fluorescerende in situ hybridisering immunostaining Live Imaging sebrafisk IL-34 macrophage
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