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Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

Article DOI: 10.3791/60168-v • 08:09 min • October 26th, 2019
October 26th, 2019 •
1,2, 1,2, 1,2, 1,2, 3, 1, 2,4, 1,2
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 2The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 3Department of Oral Biology, University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba

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Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.

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Primary Culture Neurons Embryonic Mouse Cerebellum Dissociated Cells In Vitro Culture Cellular Features Mechanisms Functions Morphology Cover Slips Poly-L-ornithine Incubator Culture Medium Seeding Medium Trypsin Solution DMEM/F12 Double Distilled Water Petri Dishes PBS HBSS Dissection Medium Uterine Horns
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