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Primær kultur af neuroner isoleret fra embryonale mus cerebellum
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Primær kultur af neuroner isoleret fra embryonale mus cerebellum

Article DOI: 10.3791/60168-v • 08:09 min • October 26th, 2019
October 26th, 2019 •
1,2, 1,2, 1,2, 1,2, 3, 1, 2,4, 1,2
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 2The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 3Department of Oral Biology, University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba

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Gennemførelse in vitro forsøg til at afspejle in vivo betingelser så tilfredsstillende som muligt er ikke en let opgave. Brugen af primær cellekulturer er et vigtigt skridt i retning af at forstå cellebiologi i en hel organisme. Den medfølgende protokol skitserer, hvordan man med held vokse og kultur embryonale mus cerebellar neuroner.

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Neurovidenskab embryo cerebellum Neuron Purkinje celle primær kultur mus
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