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Immunology and Infection

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JoVE Journal Immunology and Infection
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
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Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction

Article DOI: 10.3791/60175-v • 06:19 min • November 6th, 2019
November 6th, 2019 •
1, 1
1Section of Immunology, Vetsuisse Faculty, University of Zürich

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Summary
Automatic Translation

This protocol outlines the establishment of a mouse model for studying Malassezia-host interactions in the skin. It describes the cultivation of Malassezia in vitro, the infection of the murine skin with Malassezia, and the subsequent analysis of the inflammation and the fungal burden in the skin tissue.

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In Vitro In Vivo Mouse Model Malassezia Fungus-host Interaction Skin Disorders Atopic Dermatitis Immune Response Inflammatory Skin Diseases Skin Commensal Fungi Malassezia Inoculum MDixon Medium Erlenmeyer Flask Centrifugation PBS Pipetting
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