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Journal / Biochemistry / Kvantificering af coenzym A i celler og væv
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Biochemistry

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JoVE Journal Biochemistry
Kvantificering af coenzym A i celler og væv
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Kvantificering af coenzym A i celler og væv

Article DOI: 10.3791/60182-v • 08:51 min • September 27th, 2019
September 27th, 2019 •
1, 1, 1, 1
1Department of Infectious Diseases, St. Jude Children's Research Hospital

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Denne metode beskriver prøveforberedelse fra dyrkede celler og animalsk væv, ekstraktion og derivatisering af coenzym A i prøverne, efterfulgt af højtryksvæskekromatografi til rensning og kvantificering af det derivatiserede coenzym A ved absorbans eller fluorescens detektering.

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Biokemi coenzym A kultiverede celler animalsk væv monobromobiman højtryksvæskekromatografi solid fase ekstraktion
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