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JoVE Journal Bioengineering
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Article DOI: 10.3791/60189-v • 07:32 min • September 30th, 2019
September 30th, 2019 •
*1, *1, 1, 1
1Institute of Biomedicine & Department of Cell Biology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine
* These authors contributed equally

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Herein, we present a detailed protocol for screening small peptides that bind to FGFR2 using a phage display peptide library. We further analyze the affinity of the selected peptides toward FGFR2 in vitro and its ability to suppress cell proliferation.

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Screening Identification Small Peptides Fibroblast Growth Factor Receptor 2 Phage Display Peptide Library Antagonists Agonists Allosteric Modulators Hydro-dilution Structure Formation Target Protein High Efficient Easy To Handle Economical Commercially Available High Throughput Screening Method ITC Analysis Functional Small Peptides Ying Zhao Qiang Wang Graduate Students FGFR2 Solution Coating Buffer Concentration Micrograms Per Milliliter Square Centimeter Dish Incubate Overnight Four Degrees Celsius Shaking Blocking Solution TBST Washes
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