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Bioengineering

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JoVE Journal Bioengineering
Screening og identifikation af små peptider rettet mod fibroblast vækstfaktor Receptor2 ved hjælp af et Phage display peptid bibliotek
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Screening og identifikation af små peptider rettet mod fibroblast vækstfaktor Receptor2 ved hjælp af et Phage display peptid bibliotek

Article DOI: 10.3791/60189-v • 07:32 min • September 30th, 2019
September 30th, 2019 •
*1, *1, 1, 1
1Institute of Biomedicine & Department of Cell Biology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine
* These authors contributed equally

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Heri præsenterer vi en detaljeret protokol til screening af små peptider, der binder sig til FGFR2 ved hjælp af et fagtypning-display peptid-bibliotek. Vi analyserer yderligere affiniteten af de valgte peptider mod FGFR2 in vitro og dets evne til at undertrykke celle proliferation.

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Bioteknik lille peptid fagtypning display peptid bibliotek titer bestemmelse plaque amplifikation FGFR2 affinitet
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