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En laser Capture Microdissection protokoll som gir høy kvalitet RNA fra fersk-frosne Mouse Bones
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En laser Capture Microdissection protokoll som gir høy kvalitet RNA fra fersk-frosne Mouse Bones

Article DOI: 10.3791/60197-v • 10:11 min • September 16th, 2019
September 16th, 2019 •
1, 1, 1, 1, 1
1Department of Biomedical Research, University of Veterinary Medicine Vienna

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En LCM-protokoll (laser Capture microdissection) ble utviklet for å oppnå tilstrekkelig mengde av høykvalitets RNA for analyse av genuttrykk i bein celler. Den nåværende studien fokuserer på musen femur seksjoner. Imidlertid kan LCM protokollen som rapporteres her brukes til å studere genuttrykk i celler av noe hardt vev.

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Biologi laser fangst microdissection mus bein RNA genuttrykk RNA integritet nummer
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