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Journal / Cancer Research / Bewertung der Differenzierungskapazität von…
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Cancer Research

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JoVE Journal Cancer Research
Bewertung der Differenzierungskapazität von Mausprostataepithelzellen mithilfe der organoiden Kultur
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Bewertung der Differenzierungskapazität von Mausprostataepithelzellen mithilfe der organoiden Kultur

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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Maus-Prostata-Organoide stellen einen vielversprechenden Kontext zur Bewertung von Mechanismen dar, die die Differenzierung regulieren. Dieses Papier beschreibt einen verbesserten Ansatz zur Etablierung von Prostataorganoiden und führt Methoden ein, um (1) Proteinlysat aus Organoiden zu sammeln und (2) Organoide für die konfokale Volltonmikroskopie zu fixieren und zu färben.

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Krebsforschung Ausgabe 153 Organoid Prostata Epithel Vorläufer Basal Luminal Differenzierung Maus
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