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Cancer Research

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JoVE Journal Cancer Research
Evaluación de la capacidad de diferenciación de las células epiteliales de próstata de ratón utilizando el cultivo organoide
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Evaluación de la capacidad de diferenciación de las células epiteliales de próstata de ratón utilizando el cultivo organoide

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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Los organoides de próstata de ratón representan un contexto prometedor para evaluar los mecanismos que regulan la diferenciación. Este artículo describe un enfoque mejorado para establecer organoides de próstata, e introduce métodos para (1) recoger el lisato de proteína de los organoides, y (2) fijar y manchar los organoides para la microscopía confocal de montaje completo.

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Cancer Research Número 153 organoide próstata epitelio progenitor basal luminal diferenciación ratón
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