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JoVE Journal Cancer Research
Utvärdering av differentierings kapacitet av mus prostata epitelceller med Organoid kultur
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Utvärdering av differentierings kapacitet av mus prostata epitelceller med Organoid kultur

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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Mus prostata organoider utgör en lovande kontext för att utvärdera mekanismer som reglerar differentiering. Denna uppsats beskriver en förbättrad metod för att etablera prostata organoider, och introducerar metoder för att (1) samla in proteinlysat från organoider, och (2) Fix och fläcken organoider för hela konfokalmikroskopi.

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Cancer forskning Organoid prostata epitel progenitor basal Luminal differentiering mus
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