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Immunology and Infection

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JoVE Journal Immunology and Infection
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A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Article DOI: 10.3791/60229-v • 11:03 min • October 30th, 2019
October 30th, 2019 •
1, 1, 1, 1, 1, 1
1Immunology, Janssen Research & Development, Inc.

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A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.

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Cryo-pulverization Protocol Mouse Paws Tissue Inflammation Collagen Induced Arthritis Model Molecular Pathways Evaluation Active Pharmaceuticals Local Tissues Arthritis Research Weight-bearing Joints Mechanical Disruption Cryogenically Controlled Environment Proteomic Profiling Transcriptional Profiling Molecular Signatures Homogenous Powder Temperature Isolation Procedure High-quality RNA Molecular Characterization Research Field
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