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JoVE Journal Neuroscience
Visualizando e analisando o transporte intracelular de organelas e outras cargas em astrócitos
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Visualizando e analisando o transporte intracelular de organelas e outras cargas em astrócitos

Article DOI: 10.3791/60230-v • 07:19 min • August 28th, 2019
August 28th, 2019 •
*1, *1, 1
1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Aqui nós descrevemos um método vivo-imagem latente in vitro para visualizar o transporte intracelular das organelas e o tráfico de proteínas da membrana de plasma em astrocytes murino. Este protocolo também apresenta uma metodologia de análise de imagem para determinar itinerários de transporte de carga e cinética.

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Neurociência edição 150 astrócitos transporte intracelular imagens ao vivo cinética do tráfico endosomas degradativos connexina 43 EAAT1
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