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JoVE Journal Neuroscience
Visualisera och analysera intracellulär transport av organeller och andra laster i astrocyter
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Visualisera och analysera intracellulär transport av organeller och andra laster i astrocyter

Article DOI: 10.3791/60230-v • 07:19 min • August 28th, 2019
August 28th, 2019 •
*1, *1, 1
1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Här beskriver vi en in vitro Live-Imaging metod för att visualisera intracellulär transport av organeller och handel med plasmamembran proteiner i murina astrocyter. Detta protokoll innehåller också en bildanalys metod för att bestämma transport resvägar och kinetik.

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Neurovetenskap astrocyter intracellulär transport Live-Imaging trafficking kinetik nedbrytande endosomes Connexin 43 EAAT1
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