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Journal / Developmental Biology / Análise do metabolismo hematopoiético da pilha do …
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JoVE Journal Developmental Biology
Análise do metabolismo hematopoiético da pilha do progenitor da haste
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Análise do metabolismo hematopoiético da pilha do progenitor da haste

Article DOI: 10.3791/60234-v • 12:20 min • November 9th, 2019
November 9th, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

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As células progenitoras de tronco hematopoiético (HSPCs) transitam de um estado quiescente para um estado de diferenciação devido à sua plasticidade metabólica durante a formação sanguínea. Aqui, apresentamos um método otimizado para medir a respiração mitocondrial e a glicolíse dos HSPCs.

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Biologia do Desenvolvimento Edição 153 células progenitoras de tronco hematopoiéticas metabolismo respiração mitocondrial glicolíse análise de fluxo extracelular células não aderentes
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