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Celle type-specifik genekspression profilering i muse leveren

Article DOI: 10.3791/60242-v • 10:06 min • September 17th, 2019
September 17th, 2019 •
1, 1, 1,2
1Department of Genetics, University of Pennsylvania, 2Department of Medicine, University of Pennsylvania

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Oversættelse af ribosom affinitets rensning (Trap) muliggør hurtig og effektiv isolering af celletype specifik oversættelse af mRNA. Her demonstrerer vi en metode, der kombinerer Hydrodynamisk hale-vene injektion i en musemodel af leveren genindsættelse og fælde for at undersøge udtryks profilen for at genbefolde hepatocytter.

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Biologi lever regenerering celletype-specifik FÆLDE FÆLDE-SEQ RNA-SEQ immunopræcipitation ekspressions profilering hepatocyte hydrodynamiske hale-vene injektion
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