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Journal / Immunology and Infection / Bakteriel peptid-display til udvælgelse af nye…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bakteriel peptid-display til udvælgelse af nye Biotinylerende enzymer
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Bakteriel peptid-display til udvælgelse af nye Biotinylerende enzymer

Article DOI: 10.3791/60266-v • 10:43 min • October 3rd, 2019
October 3rd, 2019 •
1, 1,2, 1
1Department of Molecular Medicine, University of Southern Denmark, 2Department of Nephrology, Odense University Hospital

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Her præsenterer vi en metode til at vælge for nye varianter af E. coli biotin-protein Ubiquitinligase BirA at biotinylates et specifikt mål peptid. Protokollen beskriver opførelsen af en plasmid for bakteriel visning af målet peptid, generation af en BirA bibliotek, udvælgelse og karakterisering af BirA varianter.

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Immunologi og infektion instrueret Evolution tilfældig mutagenese mærkning protein engineering protein-biotin Ligase protein tag
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