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JoVE Journal Neuroscience
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups
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Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Article DOI: 10.3791/60291-v • 10:35 min • January 12th, 2020
January 12th, 2020 •
*1, *1, 2, 1, 1
1Department of Anatomy, Physiology and Cell Biology, University of California Davis, 2University of Veterinary Medicine Hannover Foundation
* These authors contributed equally

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Summary
Automatic Translation

The goal of this protocol is to isolate microvessels from multiple regions of the central nervous system of lissencephalic and gyrencephalic vertebrates.

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Microvessel Isolation Central Nervous System CNS Microvasculature Vertebrate Groups Tissue Removal Meninges Choroid Plexus Microvessel Isolation Technique Ultracentrifugation Enzymatic Dissociation CNS Tissue Dissection Lissencephalic Vertebrate Specimen MV-1 Solution Pituitary Gland Sella Turcica Vertebral Column Spinal Cord Petri Dish Dissecting Microscope
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