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JoVE Journal
Neuroscience

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使用基于 FRET 的传感器 ATeam1.03YEMK在小鼠大脑的组织切片中进行细胞内 ATP 成像
 
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使用基于 FRET 的传感器 ATeam1.03YEMK在小鼠大脑的组织切片中进行细胞内 ATP 成像

Article DOI: 10.3791/60294-v
December 19th, 2019

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Summary December 19th, 2019

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Click here for the English version.

我们描述了基于基因编码的FRET传感器ATeam1.03YEMK在小鼠前脑的有机切片培养物中细胞型特定表达的协议。此外,我们演示如何使用此传感器对神经元和星形细胞中的细胞ATP水平进行动态成像。

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