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Journal / Neuroscience / Avbildning av intracellulära ATP i Organotypic…
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Neuroscience

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JoVE Journal Neuroscience
Avbildning av intracellulära ATP i Organotypic vävnad skivor av musen hjärnan med hjälp av bandet-baserade sensorn ATeam 1,03Yemk
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Avbildning av intracellulära ATP i Organotypic vävnad skivor av musen hjärnan med hjälp av bandet-baserade sensorn ATeam 1,03Yemk

Article DOI: 10.3791/60294-v • 11:20 min • December 19th, 2019
December 19th, 2019 •
*1, *1, 1, 2, 1, 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Department of Neurology, Düsseldorf University Hospital
* These authors contributed equally

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Vi beskriver ett protokoll för cell-typ specifikt uttryck för den genetiskt kodade FRET-baserade sensorn ATeam 1,03Yemk i organotypic slice kulturer av musen framhjärnan. Dessutom visar vi hur man använder denna sensor för dynamisk avbildning av cellulära ATP nivåer i nervceller och astrocyter.

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Neurovetenskap Neurovetenskaper organotypic Brain slice astrocyte neuron AAV Hippocampus cortex FRET
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