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Journal / Immunology and Infection / Generation af in-frame-Gensletnings mutanter i…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation af in-frame-Gensletnings mutanter i Pseudomonas aeruginosa og testning for virulens dæmpning i en simpel muse model af infektion
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Generation af in-frame-Gensletnings mutanter i Pseudomonas aeruginosa og testning for virulens dæmpning i en simpel muse model af infektion

Article DOI: 10.3791/60297-v • 08:34 min • January 8th, 2020
January 8th, 2020 •
1, 1, 1,2
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University

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Her beskriver vi en enkel og reproducerbar protokol af musemodel af infektion til at evaluere dæmpning af de genetisk modificerede stammer af Pseudomonas aeruginosa i forhold til USA Food and Drug Administration (FDA)-godkendt Escherichia coli til kommercielle applikationer.

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Immunologi og infektion Pseudomonas aeruginosa genteknologi multipel gensletning markør-fri stamme validering sikkerhedsvurdering musemodel af infektion reproducerbarhed
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