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Estabelecimento e caracterização de spheroids do tumor neuroendócrino das entranhas pequenas
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Estabelecimento e caracterização de spheroids do tumor neuroendócrino das entranhas pequenas

Article DOI: 10.3791/60303-v • 09:43 min • October 14th, 2019
October 14th, 2019 •
1, 1, 2, 3, 3, 1
1Department of Surgery, University of Iowa Carver College of Medicine, 2High Throughput Screening Facility, University of Iowa Carver College of Medicine, 3Department of Pathology, University of Iowa Carver College of Medicine

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Os tumores neuroendócrinos (NETs) são originários de células neuroendócrinas da crista neural. Eles são lentos crescimento e desafiador para a cultura. Nós apresentamos uma estratégia alternativa para crescer redes das entranhas pequenas cultivando as como spheroids. Estes esferoides têm marcadores líquidos das entranhas pequenas e podem ser usados para o teste de droga.

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Pesquisa do cancro edição 152 tumores neuroendócrinos das entranhas pequenas spheroids synaptophysin Chromogranin A SSTR2 immunofluorescence Immunohistochemistry matriz extracelular rapamicina
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