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Journal / Cancer Research / 섬유 아세포 유래 3D 매트릭스 시스템은 내피 튜브 형성 분석에…
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JoVE Journal Cancer Research
섬유 아세포 유래 3D 매트릭스 시스템은 내피 튜브 형성 분석에 적용
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
Journal (Cancer Research)
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
Journal (Cancer Research)
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
 
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섬유 아세포 유래 3D 매트릭스 시스템은 내피 튜브 형성 분석에 적용

Article DOI: 10.3791/60304-v • 07:21 min • December 26th, 2019
December 26th, 2019 •
1, 2, 3, 1, 4, 1
1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet

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The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. 섬유아세포는 전처리된 배양판에 씨를 뿌리고 매트릭스 생성을 위한 아스코르브산으로 자극된다. 행렬은 탈세포화되고 관련 세포(예를 들어, 내피 세포)를 배양하도록 차단된다.

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암 연구 문제 154 종양 미세 환경 3 차원 (3D) 배양 세포 외 매트릭스 섬유 아세포 콜라겐 신혈관 발생
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