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JoVE Journal
Genetics

This content is Open Access.

解剖果蝇黑色素组飞行肌肉为奥米克斯方法
 
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解剖果蝇黑色素组飞行肌肉为奥米克斯方法

Article DOI: 10.3791/60309-v 08:33 min October 17th, 2019
October 17th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

果蝇飞行肌肉是研究转录调节、替代拼接、代谢和肌生物学的有力模型。我们提出了从活pupae中解剖荧光标记飞行肌肉的协议,以生成高富集的样品,非常适合蛋白质组学和深度测序。这些样本可以提供重要的机械见解,肌肉发育的各个方面。

Tags

遗传学, 问题 152,果蝇 ,发育生物学, 间接飞行肌肉, 活体解剖, 普皮发育, 质谱, RNA-Seq, RNA分离, 布鲁诺1, 替代拼接
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