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Journal / Genetics / Dissektion af Drosophila melanogaster Flight…
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Genetics

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JoVE Journal Genetics
Dissektion af Drosophila melanogaster Flight muskler for omics tilgange
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Dissektion af Drosophila melanogaster Flight muskler for omics tilgange

Article DOI: 10.3791/60309-v • 08:33 min • October 17th, 2019
October 17th, 2019 •
*1, *1, *1, 1,2
1Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-University Munich, 2Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Ludwig-Maximilians-University Munich
* These authors contributed equally

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Drosophila Flight muskel er en kraftfuld model til at studere transkriptional regulering, alternative splicing, metabolisme, og mechanobiology. Vi præsenterer en protokol for dissektion af fluorescerende-mærket flyve muskel fra levende pupper til at generere meget berigede prøver ideel til proteomics og dybe sekvensering. Disse prøver kan tilbyde vigtig mekanistisk indsigt i forskellige aspekter af muskel udvikling.

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Genetik Drosophila udviklingsmæssige biologi indirekte flyve muskel levende dissektion før udvikling massespektrometri RNA-SEQ RNA isolation Bruno1 alternative splejsning
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