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Journal / Genetics / Dissektion von Drosophila melanogaster Flight…
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Genetics

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JoVE Journal Genetics
Dissektion von Drosophila melanogaster Flight Muscles for Omics Approaches
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Dissektion von Drosophila melanogaster Flight Muscles for Omics Approaches

Article DOI: 10.3791/60309-v • 08:33 min • October 17th, 2019
October 17th, 2019 •
*1, *1, *1, 1,2
1Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-University Munich, 2Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Ludwig-Maximilians-University Munich
* These authors contributed equally

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Drosophila Flugmuskel ist ein leistungsfähiges Modell, um Transkriptionsregulation, alternative Spleißung, Stoffwechsel und Mechanobiologie zu studieren. Wir präsentieren ein Protokoll zur Zerlegung von fluoreszierend markierten Flugmuskeln aus lebenden Pupas, um hoch angereicherte Proben zu erzeugen, die ideal für Proteomik und Tiefensequenzierung sind. Diese Proben können wichtige mechanistische Einblicke in verschiedene Aspekte der Muskelentwicklung bieten.

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Genetik Ausgabe 152 Drosophila Entwicklungsbiologie indirekter Flugmuskel Lebendsektion Pupalentwicklung Massenspektrometrie RNA-Seq RNA-Isolation Bruno1 alternatives Spleißen
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