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Journal / Immunology and Infection / Avbildning den humana immunologiska synapsen
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Avbildning den humana immunologiska synapsen
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Avbildning den humana immunologiska synapsen

Article DOI: 10.3791/60312-v • 09:37 min • December 26th, 2019
December 26th, 2019 •
*1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 1,2, *1,2
1Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, 2Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, 3Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid
* These authors contributed equally

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Detta protokoll avbildar både den immunologiska synapsebildningen och den efterföljande polariserade sekretoriska trafiken mot den immunologiska synapsen. Cellulära konjugat bildades mellan en superantigen-pulsad Raji cell (fungerar som en antigen-presenterande cell) och en Jurkat klon (fungerar som en effektor helper T lymfocyter).

Tags

Immunologi och infektion T-lymfocyter antigen-presenterande celler immunologisk Synapse Microtubule-organiserande-Center polariserad trafik multivesikulär organ
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