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Journal / Developmental Biology / En lagdelt monteringsmetode til forlænget…
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Developmental Biology

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JoVE Journal Developmental Biology
En lagdelt monteringsmetode til forlænget tidsforskudt Konfokal mikroskopi af hele Zebrafish-embryoner
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En lagdelt monteringsmetode til forlænget tidsforskudt Konfokal mikroskopi af hele Zebrafish-embryoner

Article DOI: 10.3791/60321-v • 08:55 min • January 14th, 2020
January 14th, 2020 •
1, 2, 2, 3,4, 1,4, 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University

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Denne artikel beskriver en metode til at montere skrøbelige Zebra embryoner til forlænget tidsforskudt Konfokal mikroskopi. Denne lavpris metode er nem at udføre ved hjælp af almindelige glas bunds mikroskopi retter til billeddannelse på ethvert inverteret mikroskop. Monteringen udføres i lag af agopstået ved forskellige koncentrationer.

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Udviklingsbiologi zebrafish embryo Konfokal mikroskopi Timelapse montage transgene
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