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Immunology and Infection

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Pneumococcus-infectie van primaire humane endotheliale cellen in constante stroming

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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Deze studie beschrijft de microscopische monitoring van pneumococcus hechting aan Von Willebrand factor snaren geproduceerd op het oppervlak van gedifferentieerde menselijke primaire endotheliale cellen onder shear stress in gedefinieerde stromingsomstandigheden. Dit protocol kan worden uitgebreid naar gedetailleerde visualisatie van specifieke celstructuren en kwantificering van bacteriën door differentiële immunokleurings procedures toe te passen.

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Immunologie en infectie probleem 152 Streptococcus pneumoniae microfluïdische endotheel cellen microscopie fluorescentie shear stress hechting
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