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Immunology and Infection

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Pneumococcus infeksjon av primær Human endothelial celler i konstant Flow
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Pneumococcus infeksjon av primær Human endothelial celler i konstant Flow

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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Denne studien beskriver mikroskopisk overvåking av pneumococcus overholdelse av von Willebrand faktor strenger produsert på overflaten av differensiert menneskelige primære endothelial celler under skjær stress i definerte strømningsforhold. Denne protokollen kan utvides til detaljert visualisering av spesifikke celle strukturer og kvantifisering av bakterier ved å bruke differensial immunostaining prosedyrer.

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Immunologi og infeksjon Streptococcus pneumoniae mikrovæskebasert endothelial celler mikroskopi fluorescens skjær stress overholdelse
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