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JoVE Journal Biochemistry
Produktion, krystallisering og struktur bestemmelse af IKK-bindings domænet NEMO
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Produktion, krystallisering og struktur bestemmelse af IKK-bindings domænet NEMO

Article DOI: 10.3791/60339-v • 13:02 min • December 28th, 2019
December 28th, 2019 •
1, 1, 1, 1
1Department of Chemistry, Dartmouth College

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Vi beskriver protokoller for strukturen bestemmelse af IKK-bindende domæne NEMO af X-ray crystallography. Metoderne omfatter protein ekspression, rensning og karakterisering samt strategier for vellykket krystal optimering og struktur bestemmelse af proteinet i sin ubundne form.

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Biokemi NF-κb Nemo iκb kinase (ikk) Nemo bindende domæne (NBD) oprullet Coil X-ray crystallography protein engineering struktur bestemmelse struktur-baserede stof design
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