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Journal / Neuroscience / 从一个成年小鼠脑半球分离区域特异性微胶质，进行深度单细胞RNA测序…
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Neuroscience

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JoVE Journal Neuroscience
从一个成年小鼠脑半球分离区域特异性微胶质，进行深度单细胞RNA测序
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从一个成年小鼠脑半球分离区域特异性微胶质，进行深度单细胞RNA测序

Article DOI: 10.3791/60347-v • 09:49 min • December 3rd, 2019
December 3rd, 2019 •
1, 1
1Department of Neurobiology, Stanford University School of Medicine

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我们为从成年小鼠大脑半球的不同解剖区域分离微胶质提供方案，然后为全长转录体进行深度单细胞RNA测序的半自动库制备。该方法将有助于阐明微胶质在健康和疾病中的功能异质性。

Tags

神经科学， 问题 154， 微胶质， 隔离， 大脑区域， 荧光激活细胞排序， 单细胞RNA测序， 神经免疫学， 异质性
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