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Journal / Immunology and Infection / Tredimensionel billeddannelse af bakterieceller…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Tredimensionel billeddannelse af bakterieceller for nøjagtige cellulære repræsentationer og præcis protein lokalisering
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Tredimensionel billeddannelse af bakterieceller for nøjagtige cellulære repræsentationer og præcis protein lokalisering

Article DOI: 10.3791/60350-v • 06:33 min • October 29th, 2019
October 29th, 2019 •
2, 1, 1
1Department of Microbiology and Molecular Genetics, Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics, Princeton University

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Denne protokol forklarer, hvordan man forbereder og monterer bakterielle prøver til levende tredimensionel billeddannelse, og hvordan man rekonstruerer den tredimensionale form af E. coli fra disse billeder.

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Immunologi og infektion protein lokalisering celle form mikroskopi tredimensionel mikroskopi morfogenesis Forward convolution beregningsmæssige billedbehandling
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