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Journal / Cancer Research / 从3维球体培养中分离出类似干细胞的细胞…
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JoVE Journal Cancer Research
从3维球体培养中分离出类似干细胞的细胞
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Pancreatic Tissue Dissection to Isolate Viable Single Cells
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从3维球体培养中分离出类似干细胞的细胞

Article DOI: 10.3791/60357-v • 09:06 min • December 13th, 2019
December 13th, 2019 •
1, 1, 1, 1, 1,2,3
1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

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Summary
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我们使用人类原发性前列腺上皮细胞，报告一种新的无生物标记方法，通过基于球体的标签保留测定，对干细胞进行功能表征。介绍了 BrdU、CFSE 或远红色 2D 细胞标记的分步协议;三维球形形成;通过免疫细胞化学进行标签保留干细胞鉴定;和隔离由FACS。

Tags

癌症研究，第154期，干细胞，祖细胞，细胞分离，球体，前列腺癌，癌症标本
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