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Cancer Research

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JoVE Journal Cancer Research
Oprichting en analyse van driedimensionale (3D) organoïden afgeleid van patiënt prostaatkanker Bot metastase Specimens en hun Xenografts
Endobronchial Ultrasound-Guided Sampling of Lymph Nodes Using Transbronchial Needle Aspiration
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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
 
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Oprichting en analyse van driedimensionale (3D) organoïden afgeleid van patiënt prostaatkanker Bot metastase Specimens en hun Xenografts

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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Driedimensionale culturen van patiënt BMPC-specimens en xenografts van botgemetastaseerde prostaatkanker behouden de functionele heterogeniteit van hun oorspronkelijke tumoren, wat resulteert in cysten, sferoïden en complexe, tumorachtige organoïden. Dit manuscript biedt een optimalisatiestrategie en protocol voor de 3D-cultuur van heterogene patiëntafgeleide monsters en hun analyse met behulp van IFC.

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Deze maand in JoVE Patient Derived Organoids (BOB) Spin Down Methoden Bone Gemetastaseerde prostaatkanker (BMPC) In Vivo Stitch Imaging Immunofluorescentie Cytochemie (IFC)
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