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Journal / Cancer Research / 患者前立腺癌骨転移標本及びその異種移植片に由来する3次元(3D)オルガノイドの確立と解析…
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Cancer Research

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JoVE Journal Cancer Research
患者前立腺癌骨転移標本及びその異種移植片に由来する3次元(3D)オルガノイドの確立と解析
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患者前立腺癌骨転移標本及びその異種移植片に由来する3次元(3D)オルガノイドの確立と解析

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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患者のBMPC標本および骨転移性前立腺癌の異種移植片の三次元培養は、嚢胞、回転楕円および複雑な腫瘍様オルガノイドをもたらす元の腫瘍の機能的不均一性を維持する。本稿は、異種患者由来サンプルの3D培養とIFCを用いた解析のための最適化戦略とプロトコルを提供します。

Tags

JoVE、、患者由来オルガノイド(PDO)、スピンダウン法、骨転移性前立腺癌(BMPC)、インビボステッチイメージング、免疫蛍光性細胞化学(IFC)
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