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Journal / Cancer Research / Estabelecimento e Análise de Organoides…
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Cancer Research

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JoVE Journal Cancer Research
Estabelecimento e Análise de Organoides Tridimensionais (3D) derivados de amostras de metástase óssea do câncer de próstata paciente e seus Xenoenxertos
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Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
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Estabelecimento e Análise de Organoides Tridimensionais (3D) derivados de amostras de metástase óssea do câncer de próstata paciente e seus Xenoenxertos

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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Culturas tridimensionais de amostras de BMPC pacientes e xenoenxertos de câncer de próstata metastático mantêm a heterogeneidade funcional de seus tumores originais resultando em cistos, esferoides e organoides complexos, semelhantes a tumores. Este manuscrito fornece uma estratégia de otimização e protocolo para a cultura 3D de amostras derivadas de pacientes heterogêneos e sua análise utilizando IFC.

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Este mês em JoVE Edição 156 Organoides Derivados do Paciente (PDO) Métodos Spin Down Câncer de Próstata Metastático Ósseo (BMPC) Em Imagens de Ponto Vivo Imunofluorescência Citoquímica (IFC)
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