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JoVE Journal Cancer Research
Etablering og analyse af tredimensionale (3D) organoider afledt af patient prostatakræft Bone Metastase prøver og deres Xenografts
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Etablering og analyse af tredimensionale (3D) organoider afledt af patient prostatakræft Bone Metastase prøver og deres Xenografts

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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Tre-dimensionelle kulturer af patientenBMPC prøver og xenografts af knogle metastatisk prostatakræft opretholde den funktionelle heterogenitet af deres oprindelige tumorer resulterer i cyster, sfænoider og komplekse, tumor-lignende organoider. Dette manuskript giver en optimeringsstrategi og protokol for 3D-kultur af heterogene patientafledte prøver og deres analyse ved hjælp af IFC.

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Denne måned i JoVE Patient Derived Organoids (BOB) Spin Down Metoder Bone Metastatisk prostatakræft (BMPC) In Vivo Stitch Imaging Immunofluorescens Cytokemi (IFC)
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