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Bioengineering

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JoVE Journal Bioengineering
Fabrikation og implementering af en reference-fri trækkraft mikroskopi platform
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Fabrikation og implementering af en reference-fri trækkraft mikroskopi platform

Article DOI: 10.3791/60383-v • 08:10 min • October 6th, 2019
October 6th, 2019 •
1, 1,2,3
1Department of Biomedical Engineering, University of Delaware, 2Department of Materials Science & Engineering, University of Delaware, 3Delaware Biotechnology Institute

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Denne protokol indeholder instruktioner til implementering af multiphoton litografi til at fabrikere tredimensionelle arrays af fluorescerende fiducial markører indlejret i poly (ethylenglycol)-baserede silicagelrogeler til brug som reference-fri, trækkraft mikroskopi Platforme. Ved hjælp af disse instruktioner forenkles måling af 3D-materiale stamme og beregning af cellulære distraktionerne for at fremme målinger af trækkraft med høj gennemløb.

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Bioengineering mechanotransduction to-photon Laserscanning litografi photopatterning celle genererede kræfter biomaterialer partikel sporing hydrogel PIND stamme deformation 3D-udskrivning
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