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Journal / Cancer Research / 使用腺相关病毒-Cas9系统在Vitro建立基因工程的毛因头和颈部癌细胞系…
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Cancer Research

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JoVE Journal Cancer Research
使用腺相关病毒-Cas9系统在Vitro建立基因工程的毛因头和颈部癌细胞系
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使用腺相关病毒-Cas9系统在Vitro建立基因工程的毛因头和颈部癌细胞系

Article DOI: 10.3791/60410-v • 05:45 min • January 9th, 2020
January 9th, 2020 •
*1,2, *1,2, 3, 2,4, 1,2
1The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, 2Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Human Oncology & Pathogenesis Program (HOPP), Memorial Sloan Kettering Cancer Center, 4Institute of Pathology, Barzilai University Medical Center
* These authors contributed equally

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为了了解肿瘤，需要开发在头颈部癌症患者中发生特定基因变异的鼠模型。在这里，我们提出了一个方案，用于使用腺相关病毒-Cas9系统对原性鼠舌细胞进行体外转化，以产生具有特定基因组改变的小鼠HNC细胞系。

Tags

癌症研究， 问题 155， 头颈部癌症， 鼠癌模型， 基因组编辑， CRISPR， 腺相关病毒载体， 体外细胞转化， 肿瘤细胞系
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