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Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

Article DOI: 10.3791/60411-v • 06:27 min • February 6th, 2020
February 6th, 2020 •
1, 1, 1, 1, 1,2, 1,3
1Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, 2Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, 3Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences University of Melbourne

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Summary
Automatic Translation

Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.

Tags

Shared Blood Supply Parabiotic Mice Caudal Vein Glucose Injection Circulation Chimerism Parabiosis Model Biomedical Research Blood Chimerism Detection Safe And Simple Protocol Quick And Reliable Verification Tail Vein Injection Venous Visual Mouse Tail Fixator Anesthetized C57 Black 6 Male Mice Supine Position Electric Shaver Longitudinal Skin Incisions Subcutaneous Fascia Suture
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